• Level of study

    BAC +2

  • ECTS

    3 credits

  • Component

    Faculty of Science

Description

 

This course is a continuation of the S3 Biochemistry course. This course focuses more on the practical aspect. The principles of the usual biochemistry techniques (protein separation techniques, protein determination techniques by spectrophotometry, Western Blot/Elisa, ...) will be treated in class and then experiments related to these techniques will be carried out in practical work. It will be necessary to interpret and analyze the experiments proposed during the lab.

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Objectives

Knowledge: Understand the principles of the following techniques

- Principles of protein assays by spectrophotometry

- Principles of gel exclusion chromatography, ion and affinity exchange techniques, TLC

- Principles of protein separation techniques (SDS-PAGE gel electrophoresis).

- Western Blot and Elisa

-Know-how: Be able to carry out the experiments implementing the techniques discussed in class:

- Protein determination by spectrophotometry (bradford, lowry, biuret), standard range and quantification of a protein concentration in a sample,

- Separation of amino acids by ion exchange chromatography, identification of amino acids by TLC,

- Protein separation by SDS-PAGE gel electrophoresis 

- Preparation of several buffer solutions

-Know how to be : To have a behavior compatible with a work in binomial in TP room

- Work in pairs,

Use the work space (bench, and common work areas),

Communicate with teachers,

Respect the given working time,

Turn in work on time

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Necessary pre-requisites

Biochemistry S3

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