• Level of study

    BAC +2

  • ECTS

    4 credits

  • Component

    Faculty of Science

Description

This compulsory course allows students to consolidate the basics of biochemistry acquired in L1 by approaching this discipline through a transversal study of enzymes involved in cellular metabolism, particularly glycolysis. Several areas of biochemistry will be covered: the basics of Michaelian enzymology, the description of metabolic reactions involved in glycolysis. Finally, the technical aspect will be approached by the presentation and the analysis of the techniques allowing to measure an enzymatic activity, to purify, to quantify and to detect proteins.

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Objectives

Knowledge:

Know the basic concepts of Michael enzymology and cellular energetics in order to understand the regulation of metabolism

Basic knowledge of carbohydrate structure and metabolism, including glycolysis.

To understand that the "spontaneity" of the reactions constituting a metabolic pathway in the cells is linked to a set of intracellular (variable concentrations of the various metabolites over time) and extracellular factors (concentration of secreted metabolites, hormonal signals, ...).

-Know-how :

Locate and measure an enzymatic activity, measure a reaction rate. To measure a metabolite.

Understand and use current spectrophotometric analysis and dosing techniques, master current separation and purification methods.

Understand inter-molecular interactions.

-Know how to be :

Work in pairs,

Use the work space (bench, and common work areas),

Communicate with teachers,

Respect the given working time,

Turn in work on time.

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Necessary pre-requisites

From Molecule to Cell (L1), Biochemistry and Cell Biology (L1)

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