Training structure
Joint Continuing Education Office, Montpellier-Sète University Institute of Technology
Presentation
This short course on “Genetic Engineering” will introduce you to the use of Benchling (open-source molecular biology software for primer design and cloning prediction) as well as the entire process of PCR cloning (from primer design to obtaining bacteria capable of inducible expression of the protein of interest encoded by the plasmid obtained through cloning).
Led by Mary Arnould, a graduate of the École Normale Supérieure (ENS) in Cachan and an associate researcher at the Montpellier Institute for Infectious Disease Research (IRIM), this training program is designed for engineers, researchers, and laboratory technicians. It can address your organization’s training needs for skill enhancement or the development of new activities.
Objectives
Apply molecular engineering techniques in health biology and manipulate genomes in compliance with applicable regulations
Know-how and skills
Upon completion of the tutorials (TD) and lab sessions (TP), you will be able to:
- Design of Primers and Cloning PCR
Primer design, preparation of reagents, and programming of the thermal cycler. - Verification, Purification, and Enzymatic Digestion
Agarose gel verification, purification, and digestion of the PCR product and the vector plasmid. - Ligation, Transformation, and Colony PCR
Ligation, transformation of DH5α bacteria, and performance and analysis of colony PCR. - Culture, DNA Mini-Prep, and Protein Expression
Colony culture, DNA mini-prep and quantification, bacterial transformation, and verification of protein expression.
Program
This short course consists of 27 hours of in-person training spread over 4.5 days, including:
- 12 hours of classroom instruction
- 15 hours of hands-on training
Program
Theoretical portion (12 hours)
- Introduction to Genes and PCR
PCR amplification, primer design. - Plasmids and Enzymatic Digestion
Example of the pET30 plasmid, using restriction enzymes. - Cloning and Selection
Selection using antibiotics and the white/blue system. - DNA Ligation, Transformation, and Mini-Preparation
Ligation techniques, bacterial transformation, plasmid extraction, and analysis.
Practical component (15 hours)
Admission
Target audience
Laboratory technicians, engineers, researchers.
Tuition fees
Training fee: €2,200 (including tax)
Mandatory prerequisites
Prerequisites: Basic knowledge of DNA, the structure and transmission of genetic information in bacteria (replication, transcription, translation, genetic transfer)