Training Structure
Joint Continuing Education Office, Montpellier-Sète University Institute of Technology (IUT)
Overview
This short course on “Genetic Engineering” will introduce you to the use of Benchling (open-source molecular biology software for designing primers and predicting cloning success) as well as how to perform a complete PCR cloning process (from primer design to obtaining bacteria capable of inducibly expressing the protein of interest encoded by the plasmid obtained through cloning).
Led by Mary Arnould, a graduate of the École Normale Supérieure (ENS) in Cachan and an associate researcher at the Montpellier Institute for Infectious Disease Research (IRIM), this training program is designed for engineers, researchers, and laboratory technicians. It can be tailored to meet your organization’s training needs for skill enhancement or the development of new activities.
Objectives
Apply molecular engineering techniques in health biology and manipulate genomes in compliance with applicable regulations
Expertise and Skills
Upon completion of the tutorials (TD) and lab sessions (TP), you will be able to:
- Primer Design and Cloning PCR
Primer design, reagent preparation, and thermocycler programming. - Verification, Purification, and Enzymatic Digestion
Agarose gel verification, purification, and digestion of the PCR product and the vector plasmid. - Ligation, Transformation, and Colony PCR
Ligation, transformation of DH5α bacteria, and performance and analysis of a colony PCR. - Culture, DNA Miniprep, and Protein Expression
Colony culture, DNA miniprep and quantification, bacterial transformation, and verification of protein expression.
Program
This short training program consists of 27 hours of in-person training spread over 4.5 days, including:
- 12 hours of classroom instruction
- 15 hours of hands-on training
Program
Theoretical portion (12 hours)
- Introduction to Genes and PCR
: PCR amplification, primer design. - Plasmids and Enzymatic Digestion
Example of the pET30 plasmid and the use of restriction enzymes. - Cloning and Selection
Selection using antibiotics and the white/blue system. - DNA Ligation, Transformation, and Minipreparation
Ligation techniques, bacterial transformation, plasmid extraction, and analysis.
Practical Component (15 hours)
Admission
Target Audience
Laboratory technicians, engineers, researchers.
Tuition Fees
Training fee: €2,200 (including tax)
Mandatory Prerequisites
Prerequisites: Basic knowledge of DNA, the structure and transmission of genetic information in bacteria (replication, transcription, translation, genetic transfer)